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IMMPACT Biotechnologies GmbH
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Vector Laboratories
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Vector Laboratories
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Image Search Results
Journal: Veterinary Sciences
Article Title: Distribution of Coronavirus Receptors in the Swine Respiratory and Intestinal Tract
doi: 10.3390/vetsci9090500
Figure Lengend Snippet: Immunohistochemically stained images of corona viral receptors in tracheal sections of pigs. Representative images of formalin-fixed paraffin-embedded cross-sections of pig trachea stained with ImmPRESS VR anti-mouse IgG horseradish peroxidase (HRP) polymer detection kit (MP-6402-15; Vector Laboratories) with mouse monoclonal antibodies (1:50) specific for ( A ) aminopeptidase N (APN/CD13; sc-166105, Santa Cruz); ( B ) dipeptidyl peptidase 4 (DPP4/CD26; CACT114A-BOV2078 (The Washington State University Monoclonal Antibody Center, Pullman, WA, USA); ( C ) angiotensin-converting enzyme 2 (ACE2; sc-390851, Santa Cruz Biotechnology, Dallas, TX, USA)—note the focal expression of ACE2 on epithelial cells (arrow); ( D ) carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1/CD66a; sc-166453, Santa Cruz) receptors; ( E ) secondary antibody control and ( F ) blank. Brown represents a positive expression of the antibody, and the nucleus counterstained with hematoxylin is blue. Scale bar—100 μm.
Article Snippet:
Techniques: Staining, Formalin-fixed Paraffin-Embedded, Plasmid Preparation, Expressing